Journal: European journal of cell biology
Article Title: Human pluripotent stem cell-derived intestinal organoids for pharmacokinetic studies.
doi: 10.1016/j.ejcb.2025.151489
Figure Lengend Snippet: Fig. 1. Differentiation of human iPSCs into intestinal organoids (IOs). (A) A schematic drawing of the overall culture timeline of iPSCs to establish IOs, showing respective culture platforms, matrices, and key molecules at each stage of differentiation. (B) Bright-field images of iPSC-derived differentiated cells. (Upper) Bright- field images showing iPSCs in 3D cluster microwell culture (day 0, 3, 8) (Representative 2 wells are shown). (Lower) iPSC-derived IOs (iPSC-IOs) formed in Matrigel- based culture on differentiation days 15, 22, and 29. (Right table) Cell growth rates in passage 1 (P1) on day 22 (d22) and P2 on d29 are shown as fold-change in cell numbers after 7 days of culture versus the starting cell no. of 1.0 × 105 cells. Scale bars: 200 μm (upper), 500 μm (lower). (C) Relative gene expression level of marker genes analyzed by qPCR on day 0 (before differentiation), day 3 (endoderm), day 8 (mid-/hind-gut), day 15 (P0), day 22 (P1), and day 29 (P2) are shown. The qPCR data are normalized against the adult small intestine (ASI = 1). Data are expressed as the mean ± SD (standard deviation), N = 3. Significances are shown as *p < 0.05, or **p < 0.01, by Dunnett’s multiple comparisons tests compared with control ASI in each gene set.
Article Snippet: Total RNA of the human adult small intestine (ASI) (Takara Bio, Cat. No. 636539, Lot.
Techniques: Derivative Assay, Gene Expression, Marker, Standard Deviation, Control